![]() Conclusion: DNeasy plant mini kit is an efficient for genomic DNA extraction and purification. L-10 shows the highest yield of DNA that is 462.47 and the lowest DNA yield is of L-7 that is 355.54, the OD260/280 of L-3 is high then the remaining nine lines. Purified genomic DNA has A260/A280 ratios of 1.5–1.77, and absorbance confirming high purity. Results showed the differences in DNA yield and DNA quality among the advanced lines of ten wheat samples. DNA concentration was also measured and quantified by ultraviolet spectrophotometer using ultraviolet spectrophotometer. RNA and other polysaccharides proteins contamination in the Data is not recoded providing a very rapid and sensitive means of estimating the nucleic acid concentration. After the extraction of genomic DNA from the plant tissues the quantification, quality and concentrations of isolated genomic DNA were verified on 1% of Agarose gel. Results: The results literally focused on to study an efficient method for extraction of genomic DNA and to determine the quality and quantity of genomic DNA. Agarose gel was run after the DNA extraction and then the Spectrophotometric Determination was done. Genomic DNA was extracted from these leaves using DNeasy plant mini kit. When the plants achieved optimum size after germination, the flag leaf were collected on the basis of weight and stored at -80 for molecular analysis. ![]() The sterilized seeds were cultured in Petri dish and kept it in growth chamber at 25oC. Again washed with mercuric chloride and washed with double distilled water for three times. Methodology: Ten advance lines of wheat were surface sterilized by 70% ethanol for two minutes and then rinse with double distilled water three times. The results obtained are from the original project of Syed Tariq Shah, Senior Scientist, NIFA Peshawar. The internship was completed at NIFA (Nuclear Institute of Food and Agriculture), Peshawar, KP-Pakistan under the supervision of Syed Tariq Shah, Senior Scientist, NIFA Peshawar in 2014. The maximum yield plant-1 was recorded in local selection (265.43 g) followed by Black Mission having (256.2 g) plant-1.The result of this study suggested that brown turkey followed by local selection was found the best in relation to growth and yield of fig tree in Agro-climatic condition of Islamabad.īackground: The manuscript is originally the internship report of Muhammad Junaid, submitted for the partial fulfilment of the degree of BSc (h) to University of Swabi. Maximum TSS of fruits was observed in Black Mission (12.49) while minimum TSS (11.03) of fruits was recorded in Brown Turkey. Fruit volume, and maximum fruit size was observed in Brown Turkey (22 ml) followed by Black Mission having (19.33 ml). Maximum fruit weight (25.77 g) was recorded in Local selection while lowest fruit weight (20.39 g) was found in Brown Turkey cultivar. Highest number of fruits plant-1 were observed in Brown Turkey (12.48) followed by Black Mission, while lowest number of fruits plant-1 (10.38) was noted in local cultivar. It was observed that number of fruits plant-1 indicated a significant difference among cultivars. ![]() The experiment was laid out in Randomized Complete Block Design (RCBD). Five parameter (number of fruits, weight, size (volume), TSS, and yield plant-1) with three replications and five plants replication-1 for each cultivars were studied during the experiment and after harvesting. ![]() Production and quality data were recorded for the evaluation of these cultivars. The present study was undertaken to investigate “the performance of fig cultivars (Local, Brown Turkey and Black Mission) planted in high density system under agro-climatic conditions of Islamabad”, at the experimental farm of National Agriculture Research Centre (NARC) Islamabad. Fig (Ficus carica) is one of the famous fruit species in world wide. ![]()
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